Fig 5.

Effect of rearing temperature on larval European eel (Anguilla anguilla) gene expression of gh (A) or igf-1 (G-H) at specific developmental Stages (1, 2 and 3) and gh (B) or igf-1 (I) in real time, as well as effect of age on gh (C-F) or igf-1 expression (J-M) at each temperature. Relationships between age and gh expression can be explained by cubic sigmoidal regressions at all temperatures (P < 0.0001, R² > 0.82). The relationship between age and igf-1 expression can be explained by cubic sigmoidal regressions at 16 or 18°C and by linear regressions at 20 or 22°C (P < 0.002, R² > 0.64). Data points with an asterisk (*) were not included in the statistical model due to insufficient sample size. Values represent means (± SEM) among four crosses at each temperature and treatments with the same letters are not significantly different (P > 0.05).