Fig 7. qPCR-RT measurement of RNA (cDNA) expression for coded proteins involved in inflammation, extracellular matrix regulation, and heart metabolism.

(B), (C), (F), and (I) represent those genes (Timp2, Timp3, Fn1, Pdk1, respectively) whose expression is downregulated by IGF-1 deficiency and recovered by substitutive treatment; (A), (G), and (H) refer to those genes (C3, Aqp4, Ahsg, respectively) underexpressed in both Hz groups; (D) shows that Serpine1 was overexpressed in Hz animals and contrarily underexpressed when substitutive treatment was applied; and (E) illustrates how Serpinh1 is upregulated by IGF-1 deficiency and how IGF-1 substitutive therapy normalizes its expression (n = 10 animals per group). *p<0.05, ** p 0.01 vs. controls (Wt group); & p<0.05 Hz+IGF-1 group vs. untreated Hz mice.