Table 2.
Self-renewal (Mouse)
| Reagent | Company | Cat no | Final concentration |
|---|---|---|---|
| Advanced DMEM | ThermoFisher Scientific, Invitrogen | 12634–010 | n/a |
| Penicillin/Streptomycin | ThermoFisher Scientific, Invitrogen | 15140–122 | 100 U/ml (Pen) 100 μg/ml (Strep) |
| Hepes | ThermoFisher Scientific, Invitrogen | 15630–056 | 10 mM |
| Glutamax | ThermoFisher Scientific, Invitrogen | 35050–038 | 2 mM |
| N2 | ThermoFisher Scientific, Invitrogen | 17502–048 | 1:100 |
| B27 (-Vit A) | ThermoFisher Scientific, Invitrogen | 12587–010 | 1:50 |
| N-acetylcysteine | Sigma-Aldrich | A9165 | 1.25 mM |
| Matrigel | Corning | 356231 | Undiluted |
| R-spondin conditioned medium | Stem Cell Institute, University of Cambridge | From 293T-HA-Rspo1-Fc cell line made by Calvin Kuo, Stanford | 5% v/v |
| mEGF | R and D Systems | 2028-EG-200 | 50 ng/ml |
| Noggin | R and D Systems | 6057 NG-100 | 100 ng/ml |
| FGF10 | R and D Systems | 345-FG-025 | 100 ng/ml |
| mFGF7 | R and D Systems | 5028 KG_025 | 100 ng/ml |
| CHIR 99021 | Stem Cell Institute, University of Cambridge | n/a | 3 μM |
| SB 431542 | Tocris | 1614 | 10 μM |