Figure 5.

PDGFRα directly binds c-Kit and stimulates the expression of SCF. (A) Cardiac fibroblasts were treated with PDGF-AA (10 ng/mL) for 24 hours. Lysates from the cardiac fibroblasts were immunoprecipitated with an anti-PDGFRα antibody or a nonspecific immunoglobulin G control (IgG), followed by immunoblotting with the anti-c-Kit antibody or anti-PDGFRα antibody. Protein input served as an immunoblotting control (input). (B) Cardiac fibroblasts were treated with PDGF-AA for 12 hours. The mRNA expression of SCF was tested by RT-qPCR. IP: immunoprecipitation; IB: immunoblotting. The data are representative of three independent experiments. Data are expressed as mean ±SD. *** Indicated p<0.001.