Table 2.
Method | Mechanism | Input volume | Virion co-purification |
---|---|---|---|
Differential ultracentrifugation | Density and size | 35 ml | Yes |
ExoQuick (SBI Biotech Inc.) | Precipitation | 250 μl | Yes |
Total EV (Invitrogen Inc.) | Precipitation | 250 μl | Yes |
PEG-2000 | Precipitation | 250 μl – 250 ml | Yes |
CD63 magnetic beads | Bead-based surface marker | 1 – 35 ml | No |
Composite magnetic beads | Bead-based surface markers (n=5 markers) | 100 μl – 1ml | No |
Size exclusion chromatography | Size-bases isolation | 100 μl – 35 ml | Yes |
Density flotation (for example, Iodixanol) | Density | 35 ml | Yes |