Table III.
Comparison of p53RE qHTS assay activity calls and QSAR model predictions for bacterial mutagenicity or traditional genotoxicity assay results
| QSAR | Traditional genotoxicity assays | ||||
|---|---|---|---|---|---|
| BM (CASE Ultra QSAR) | BM (Leadscope QSAR) | BM (no S9) | CA (no S9) | MN (adjusted) | |
| Sensitivity | 0.11 [0.09 – 0.12] | 0.11 [0.09 – 0.13] | 0.12 [0.09 – 0.15] | 0.11 [0.08 – 0.15] | 0.17 [0.1 – 0.24] |
| Specificity | 0.97 [0.96 – 0.97] | 0.97 [0.96 – 0.98] | 0.96 [0.94 – 0.97] | 0.96 [0.94 – 0.98] | 0.96 [0.93 – 0.98] |
| PPV1 | 0.48 [0.42 – 0.53] | 0.53 [0.46 – 0.59] | 0.68 [0.58 – 0.78] | 0.71 [0.57 – 0.83] | 0.55 [0.39 – 0.71] |
| NPV2 | 0.78 [0.77 – 0.79] | 0.78 [0.76 – 0.79] | 0.6 [0.57 – 0.63] | 0.55 [0.51 – 0.59] | 0.79 [0.75 – 0.82] |
| True positive | 136 | 121 | 60 | 36 | 21 |
| False positive | 149 | 108 | 28 | 15 | 17 |
| False negative | 1147 | 997 | 430 | 289 | 100 |
| True negative | 4153 | 3465 | 632 | 356 | 377 |
Positive predictive value
Negative predictive value
True positive: chemicals active in both the p53RE assay and the specific traditional genotoxicity assay
False positive: chemicals active in the p53RE assay but inactive in the specific traditional genotoxicity assay
False negative: chemicals inactive in the p53RE assay but active in the specific traditional genotoxicity assay
True negative: chemicals inactive in both the p53RE assay and the specific traditional genotoxicity assay