Figure 2.

pH‐sensitivity of channel gating of DrHv1. (A) Representative proton current traces recorded from a single DrHv1‐expressing HEK293T cell. The holding potential was −80 mV. A series of 3‐s depolarizing voltage pulses were stepped from −80 mV to 100 mV. pH _i was 7.0; pH _os were 7.5, 7.0, 6.5, and 6.0. (B) Normalized current–voltage relationships of DrHv1‐derived proton currents recorded at different ΔpHs (n = 5). pH _i = 7.0; pH _os were 7.5 (empty circle), 7.0 (filled squares), 6.5 (empty triangles), and 6.0 (filled triangles). (C) Conductance–voltage relationships of DrHv1 at different ΔpHs (n = 5). Data were taken from the same set of cells as in (B).