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. 2017 Aug 7;5(15):e13350. doi: 10.14814/phy2.13350

Figure 4.

Figure 4

Effects of intermittent heat stress and/or 14 days of denervation on the protein expression levels in sham‐operated and denervated soleus muscles. The phosphorylated (p) and total (t) levels of protein kinase B (Akt), ribosomal protein S6 (S6), and AMP‐activated protein kinase α (AMPK α); the expression level of 70‐kDa heat‐shock protein (HSP70), peroxisome proliferator‐activated receptor‐γ coactivator‐1α (PGC‐1α), ubiquitinated proteins, and glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) were evaluated by immune blotting. Representative results of immunoblotting are shown in A and N. The expression level of each protein was calculated after normalization using the expression levels of GAPDH (B, C, E, F, H, I, K, L). The phosphorylation rate of the protein was calculated by dividing the expression level of phosphorylated protein by the expression level of total protein (D, G, M). The relative values pertaining to the sham‐operated Cont muscles are also shown. Means ± SD (n = 8 per group). H and D: main effect of heat stress and denervation, respectively; H×D: interaction of effects of heat stress and denervation; *: the result of Tukey's multiple comparisons test was P < 0.05. See Figure 2 for other abbreviations.