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. 2017 Aug 7;5(15):e13350. doi: 10.14814/phy2.13350

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© 2017 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Effects of intermittent heat stress and/or 14 days of denervation on the transcription of atrogin‐1/muscle atrophy F‐box (Atrogin‐1, A) and muscle RING‐finger protein‐1 (MuRF‐1, B) genes in soleus muscles were evaluated by real‐time polymerase chain reaction (RT‐PCR). The expression levels of Atrogin‐1 and MuRF‐1 mRNA were obtained by calculation after normalization using the expression level of TATA box‐binding protein (Tbp). The relative expression levels pertaining to the sham‐operated Cont muscles are also shown. Mean ± SD (n = 8 per group). D: main effect of denervation, respectively; H×D: interaction of effects of heat stress and denervation; *: the result of Tukey's multiple comparisons test was P < 0.05. See Figure 2 for other abbreviations.