Figure 1.

The dietary intake of mice in each treatment, and their effect on major bronchoalveolar lavage fluid cell subtypes. In (A), female BALB/c mice (dams) were fed vitamin D‐supplemented (VitD⁺) or vitamin D‐nonsupplemented (VitD⁻) diets from 3 weeks of age and used to produce offspring. A subgroup of vitamin D‐deficient offspring were fed a vitamin D‐supplemented diet from 8 weeks of age (VitD^−/+). In (B), total, macrophage and neutrophil cell numbers in bronchoalveolar lavage fluid (BALF) from these offspring at 12 weeks of age are shown with macrophages and neutrophils identified in a representative cytospin of BALF (×100 magnification). Data are shown as mean ± SD (*P < 0.05; two‐way t test) for ≥18 mice per treatment. The results for macrophage and neutrophil numbers were partially reported in a previous publication (Roggenbuck et al. 2016) with an additional 3–5 mice per treatment added from a new experiment.