Figure 5.

Vitamin D deficiency reduced the activation status of B cells in the airway‐draining lymph nodes. Female BALB/c mice (dams) were fed vitamin D‐supplemented (VitD⁺) or vitamin D‐nonsupplemented (VitD⁻) diets from 3 weeks of age and used to produce adult offspring. In (A), the proportion of B cells was determined using flow cytometry to select for MHC class II+ and B220+ cells, with the proportion of B cells (MHC class II+B220+) for each mouse shown in (B). In (C), CD86 expression is depicted for representative samples, with the level of expression of CD86 on MHC class II+B220+ cells shown in (D). Data are for 5–6 mice per treatment (mean ± SD, *P < 0.05, two‐way t test).