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. 2001 Aug 7;98(17):9954–9959. doi: 10.1073/pnas.171098398

Figure 3.

Figure 3

PC dendritic spines and their innervation. (a, b, and c/c1) Synaptic profiles of the three types of synapses of the PC dendrites in a vehicle-treated cerebellum labeled with GABA Ab. PF synapses on branchlet spines (a), CF synapses on spines of the proximal dendrites (b), and synapses made by the inhibitory neuron on the smooth dendritic shaft (c/c1). Note GABA Ab labeling is present only in the third type of synapse. (d) PF synapse made onto branchlet spines under TTX. (e–h) Synapses made on spines of the proximal dendrites after TTX treatment. Spines contacted by terminals of a PF (e/e1), a CF (f/f1), and an inhibitory neuron (g/g1 and h). In h, the inhibitory terminal makes a synapse also with the dendritic shaft. Note that in e1, the lack of GABA immunoreactivity is accompanied by reactivity in another element (e2). (e1–h1) Higher magnification of the boxes outlined in the above-located pictures to show more clearly that the GABA labeling is absent in the terminals of e1 and f1 and present in those of g1 and h1. [Bar = 0.35 μm (for a–h) and 0.13 μm (for c1 and e1–h1).] For a higher magnification of a, b, and d, see Fig. 8, which is published as supplemental data on the PNAS web site.