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. 2017 Aug 14;7:8095. doi: 10.1038/s41598-017-08116-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of BR on seed longevity. (a) Survival rates of wild type Col-0 non-primed seeds or seeds primed with 0, 0.2, 2 or 20 μM EBL after 0 or 3 days of CDT. (b) Survival rates of wild type Col-0 non-primed seeds or seeds primed with 0, 1, 5, 10 or 50 μM Brz after 0 or 3 days of CDT. (c) Germination of wild type Col-0, Est-1, cyp85a1 cyp85a2 (cyp85a1/a2) and det2 after priming and subsequent CDT for 3 or 5 days. Photos were taken after 7 days of imbibition. (d) Survival rates of wild type Col-0, Est-1, cyp85a1/a2 and det2 after priming and subsequent CDT for 0, 3 or 5 days. (e) Germination speed of wild type Col-0, Est-1, cyp85a1/a2 and det2 before (non-primed; upper panel) and after (primed; lower panel) without CDT. For (a), (b), (d) and (e), values presented are means ± SD of three replicates. Wild type Col-0 (Col-0). Different letters in (a), (b) and (d) indicate significant differences (P < 0.05, Tukey-Kramer tests).