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. 2017 Aug 14;7:8097. doi: 10.1038/s41598-017-08232-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Generation of iSkM progenitor cells by exogenous expression of Calcein^low SCs-enriched TFs from MEFs. (a) Representative morphology of iSkM progenitor cells generated from MEFs. Low-power field of iSkM progenitor cells (left; scale bar: 500 µm) and high-power field of MEFs, iSkM progenitor cells, and cultured SCs (right; scale bar: 200 µm). n > 3. (b) Representative western blot showing overexpression of Pax3, Pax7, Mef2b, and Pitx1 by retrovirus (left) and dox-inducible lentivirus vectors, respectively (dox concentrations was 0, 1, 3, 10, 30 ng/ml). O.E., overexpression. n = 3. (c) Analysis of TFs essential for generating iSkM progenitor cells by retrovirus vector. (d) The incidence of iSkM progenitor cells by dox-inducible lentivirus vector. (e) Treatment with LIF enhanced the production efficiency of iSkM progenitor cells. Number of established lines/number of trials is indicated on the upper part of bar in (c,d and e).