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. 2017 Aug 14;7:8063. doi: 10.1038/s41598-017-07941-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Combining CpdA with DEX results in a distinct GR cofactor recruitment profile. (a) MARCoNI assay result with recombinant GRα-LBD-GST stimulated with DEX (1 µM) and/or CpdA (10 µM). Interaction between the recombinant GR and the immobilized cofactor-derived peptides are detected with an anti-GST antibody labeled with Alexa 488, after several washing steps to eliminate unbound GR proteins. The top 10 interacting peptides with DEX-bound GR-LBD are shown here. Data was normalized via LOESS regression (Mean + SEM, n = 3). (b) Detail result of (a): SHP (=NR0B2) peptide interacts stronger with CpdA/DEX-stimulated GR compared to DEX-bound GR. Statistical analysis via one-way ANOVA (p-value < 0.001) with post-hoc t-test (corrected for multiple testing) (***p < 0.001).