Figure 1.
Stress-based antibiotic susceptibility testing method. Shear flow potentiates the action of antibiotics against bacteria covalently-bound to the floor of a microfluidic channel. Bacterial death is monitored over time via automated microscopy. A fluorescent dye gives the number of dead bacteria while phase contrast images show the total bacterial count. If the percent of dead bacteria is >6% at the end of the assay, the strain is susceptible to the antibiotic of interest.