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. 2017 Aug 14;7:8031. doi: 10.1038/s41598-017-07584-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sample image analysis of GEN-susceptible E. cloacae. (A) Raw phase contrast image at t = 120 min. (B) Corresponding raw fluorescence image at t = 120 min. (C) Composite image showing the identified bacteria in the phase contrast (white marks) and fluorescence (red marks) images. N_F: number of fluorescing cells; N_P: total number of cells.