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. 2017 Aug 14;7:8109. doi: 10.1038/s41598-017-05451-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Increased levels of Notch signalling pathway components in Usp9x ^−/Y neocortices. (A) Immunoblot analysis showing increased levels of Notch intracellular domain (NICD) in E12.5 and E14.5 Usp9x ^−/Y neocortices (n = 3). (B) Quantification of relative NICD protein levels in (A) after normalisation to β-tubulin. (C) qRT-PCR analyses showing slightly increased Hes1 and Hes5 gene expression in E12.5, E14.5 and E16.5 Usp9x ^−/Y neocortices (n = 3). (E) Immunoblot analysis showing increased BLBP protein levels in E12.5, E14.5 and E16.5 Usp9x ^−/Y neocortices (n = 3). (F) Quantification of relative BLPB protein levels in (E) after normalisation to β-tubulin. (G) Coronal sections of E12.5 Usp9x ^+/Y and Usp9x ^−/Y neocortices stained with Nestin and BLBP (n = 3). BLBP displayed higher immunoreactivity in Usp9x ^−/Y neocortices (c,d) No difference was observed for Nestin expression (a,b). Scale bars = 80 μm. LV- lateral ventricle, VZ- ventricular zone. *p < 0.05.