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. 2017 Aug 14;7:8077. doi: 10.1038/s41598-017-07560-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Figure-2 — TET1 mRNA and protein expression is increased in cells exposed to 3% O₂. JEG3 cells were cultured in 3% or 21% O₂ for 24 h, 48 h and 72 h. TET1 (A) and TET2 (B) mRNA expression was analyzed using qPCR. (*P < 0.05, **P < 0.01, one-way ANOVA) (C) The protein expression of HIF1α, HIF2α, TET1 and TET2 was detected by western blotting. α-Tubulin was used as the internal control.