Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 14;7:8077. doi: 10.1038/s41598-017-07560-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Figure-4 — The migration and invasion capacities of JEG3 cells are more significantly suppressed by TET1 knockdown in cells exposed to 3% O₂ than in cells exposed to 21% O₂. (A,B) Real-time q PCR and western blot analyses showing the specific knockdown of TET1 in JEG3 cells by shRNA. (C) The migration and invasion capacities of JEG3 cells transfected with scramble shRNA or TET1 shRNA and then exposed to 21% or 3% O₂ were assessed using the transwell assay. (D) The migrated or invaded cells were counted and the quantitative data are expressed as the fold change from the control of three independent experiments. (*P < 0.05, **P < 0.01, shCtrl vs. shTET1; ^##P < 0.01 compared to shCtrl in 21% O₂; there was no significant difference between the shTET1 cells exposed to 3% and 21% O₂, one-way ANOVA) (E) Proliferation capacity of JEG3 cells transfected with scramble shRNA or TET1 shRNA and then exposed 3% O₂. (P = 0.0.84, between the shCtrl cells exposed to 3% and 21% O₂, one-way ANOVA).