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UL49.5 targets TAP for degradation by proteasomes. EBV-transformed B cells transduced with retroviruses encoding UL49.5 in the antisense (MoDo) or sense (MoDo UL49.5) orientation were metabolically labeled and chased in the absence or presence of the proteasome inhibitor Cbz-L3 for the times indicated. Transferrin receptor (Tfr), TAP, and UL49.5 were immunoprecipitated from cell lysates and analyzed by SDS/PAGE.
