Figure 5.

sPLA₂-HDL inhibits Ca²⁺ flux in platelets. Baseline Ca²⁺ levels were measured by flow cytometry for 1 min and then platelets were treated either with vehicle, nHDL (50 µg/mL), sPLA₂-HDL-low (50 µg/mL), sPLA₂-HDL-high (50 µg/mL), sPLA₂ or varespladib for 2 min. Ca²⁺ flux was subsequently induced with ADP as indicated by the arrow (10 µM). (a) Values are normalized to the baseline and expressed as maximal Ca²⁺ flux upon ADP stimulation. Results are shown as mean ± SEM (n = 4). Statistical significance was assessed by one-way ANOVA followed by Dunnett’s post hoc test. *p < 0.05 versus vehicle-pretreated and ADP-stimulated platelets. (b) One representative recording of Ca²⁺ flux is shown.