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. 2017 Aug 14;7:8090. doi: 10.1038/s41598-017-08649-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of pitavastatin-zoledronic acid combinations on caspase activity. Caspase 8 (A), 9 (B), and 3/7 (C) activity of A2780, Skov-3 and Ovsaho cell lines were measured by Caspase-Glo assays. Cells were treated with the indicated concentrations of pitavastatin and zoledronic acid for 48 hr. The effect of the drug combinations were significantly different to the effect of pitavastatin alone where shown (mean ± SD; N = 3; *P < 0.05, ** < 0.01; paired t-test). PARP and PARP cleavage (C) were measured by western blot analysis (n = 3).