Figure 4.

The effect of the pitavastatin-zoledronic acid drug combination is blocked by geranylgeraniol. (A) Cells treated with pitavastatin (A2780 and Ovsaho 1 µM and Skov-3 5 µM) and farnesol (10 µM) geranylgeraniol (10 µM) zoledronic acid (10 µM) for 48 hrs. PARP cleavage was assessed by immunoblotting. The results are representative of 3 experiments. (B) Skov-3 cell line was treated with pitavastatin or pitavastatin and zoledronic acid (10 µM) and geranylgeraniol (10 µM) and farnesol (10 µM) and after 72 hrs relative cell number was determined by staining with SRB. (C) morphological changes of Skov-3 cell line treated with indicated drug concentration for 72 hr were visualized by phase - contrast light microscopy.