Figure 11.

Histopathological analyses of mitosis and apoptosis in the tumors of SKOV-3 xenografted nude mice followed by CoQ₀ treatment. (A,B) Histopathological staining was performed to detect the mitotic- and apoptotic-cells in SKOV-3 xenografted tumors following CoQ₀ (1.5 and 2.5 mg/kg) treatment using light microscopy (20 × and 200 × magnification). Arrows indicate mitotic (tumor control) and pyknotic tumor cells (CoQ₀). The number of mitotic-positive and apoptotic-positive cells in microscopic fields of 3 samples was averaged, and presented as histogram. (C,D) In situ detection of apoptosis was performed using TUNEL staining in tumor sections from control and experimental analogues treated with CoQ₀ (1.5 and 2.5 mg/kg). The number of TUNEL-positive cells were quantified from 3 microscopic fields, and averaged. Results expressed as mean ± SD of three independent assays (n = 3). Significant at *p < 0.05; **p < 0.01; ***p < 0.001 compared with vehicle treated mice.