Fig. 2.

Confocal microscopic study of CAG7:8 IBCs. Sixteen-week-old gnotobiotic tox176 mice were killed after a 4-week infection with CAG7:8. Sections were processed exactly as in Fig. 1D.(A) A spherical IBC (red). (Insets) A single section stained with antibodies H. pylori (red) and the late endosomal marker, Lamp1 (blue). The results reveal bacterial cells residing within this endosomal compartment. (B) IBC with a dispersed phenotype in a host cell that has developed punctate disruptions of its plasma membrane (the affected cell is also shown in the Inset). (C) CAG7:8 is seen traversing a breach in its host epithelial cell plasma membrane (arrow). 3D reconstructions of serial confocal scans of the sections presented can be found in Movies 2–4, which are published as supporting information on the PNAS web site. [Bars, 10 μm (5 μm in B Inset).]