Fig. 3.

Intracellular collections of H. pylori form in actively dividing GEPs. Six- to 10-week-old tox176 mice were infected for 4 weeks with CAG7:8 and given an i.p. injection of BrdUrd (blue) 1.5 h before death. Cryosections from the zymogenic region of their glandular epithelium were stained with antibodies to H. pylori (red) and E-cadherin (green) and then serially scanned with a confocal microscope at an optical plane thickness of 0.4–0.6 μm. (A and B) S-phase epithelial cells containing small IBCs. Images were taken from 3D reconstructions of serial confocal scans (see Movies 5 and 6, which are published as supporting information on the PNAS web site). A z axis scan of the IBC portrayed in B can be found in Movie 7, which is published as supporting information on the PNAS web site. (C) Conventional photomicrograph of a section containing BrdUrd-positive (blue) and MAA-positive (magenta) GEPs with intracellular CAG7:8 (red). GEP membrane-associated β-catenin appears green. (D) Confocal microscopic image of a MAA-positive (blue) GEP (membranes appear green after staining with antibodies to β-catenin), harboring H. pylori (red). See Movie 8, which is published as supporting information on the PNAS web site, for the corresponding 3D reconstruction. (Bars, 10 μm.)