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. 2017 Aug 14;7:8060. doi: 10.1038/s41598-017-08868-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression profiles of PHF20 during osteoblast differentiation. Osteoblast lineage MC3T3-E1 and C3H10T1/2 cells were differentiated in osteogenic medium (OM) for 0, 2, 4, and 8 days. Expression levels of PHF20 and osteoblast differentiation markers were determined by RT-PCR (A,C), qRT-PCR (B), and Western blot (D). β-actin was used as internal control. Arrows (→) (A,C,D) indicate cropping lines and gels/blots were obtained under the same experimental conditions. Values are expressed as means ± SEM (n = 3). *p < 0.05, **p < 0.01 and ***p < 0.001 compared to Day 0.