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. 2017 Aug 14;7:8091. doi: 10.1038/s41598-017-08547-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of PT treatment on the viability and morphology of PC9 and A549 cells. Both cell lines were treated with increasing concentrations of PT (20, 40, and 60 μM) and assessed at time points of 24 and 48 h. The cell viability was analyzed by CCK-8 and expressed as OD values (% Control). The morphology of both cells were observed under an inverted phase-contrast microscope after the cells were treated for 24 h, and images were obtained. Significant cell shrinkage and a decreased cellular attachment rate were observed in the PT treatment groups with a dose dependent manner. All of the results were expressed as the mean ± SD; n = 6. ^aP < 0.05 vs. the control group, ^bP < 0.05 vs. the 20 μM PT-treated group, ^cP < 0.05 vs. the 40 μM PT-treated group.