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. 2017 Aug 14;7:8091. doi: 10.1038/s41598-017-08547-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of PT treatment on the depolarization of mitochondrial membrane, Caspase 3 activity, ROS generation, and GSH level in PC9 and A549 cells (24 h). (A) Representative merged images of JC-1 red (normal MMP)/green (low MMP) cells, and the MMP levels were expressed as the proportion of cells with a low MMP. (B) Intracellular Caspase 3 activity; (C) ROS concentration; (D) Intracellular GSH level; and those three indexes in the control group were defined as 100%. All of the results were expressed as the mean ± SD; n = 6. ^aP < 0.05 vs. the control group, ^bP < 0.05 vs. the 20 μM PT-treated group, ^cP < 0.05 vs. the 40 μM PT-treated group.