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. 2017 Aug 14;7:8091. doi: 10.1038/s41598-017-08547-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of PT treatment combined with CHOP siRNA on cell viability, Caspase 3 activity, ROS generation, CHOP and apoptosis-related protein levels in PC9 and A549 cells (24 h). (A) Cell viability; (B) Intracellular Caspase 3 activity; and (C) ROS concentration were shown, and those three indexes in the control group were defined as 100%. (D) Representative western blot results of the key proteins, including CHOP, Bcl2, Bax, Caspase 3, and p53 were shown. Membranes were re-probed for β-actin expression to show that similar amounts of protein were loaded in each lane. The results were expressed as the mean ± SD; n = 6. ^aP < 0.05 vs. the control group, ^bP < 0.05 vs. the CHOP siRNA-treated group, ^cP < 0.05 vs. the Control siRNA + 40 μM PT-treated group.