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. 2017 Aug 14;7:8012. doi: 10.1038/s41598-017-06855-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Profiling of the phospholipids in L. innocua NCTC 11288 at different stages of the cell cycle. Panel A shows representative ³¹P NMR spectra of the lipid fraction collected from B period cultures and cultures at the boundary of the C and D periods. The relative area of the integrations of the appropriate resonance(s) were taken as a fraction of the total integrations for each spectrum and used to generate the fraction size given for each sample. The shift of phosphate mono-ester-containing lipids such as PA, is pH dependent. PE exhibits several resonances due to concentration and pH-dependent solvent interactions^{15, 78}. Panel B shows the abundance of lipids in the B period and at the C/D period boundary from cells collected in the exponential phase. Asterisks mark the head groups (CL, PE) for which the difference in abundance is considerable (when comparing standard deviations, n = 5 independent samples profiled using quantitative ³¹P NMR). CL, cardiolipin; lyso-PA, lyso-phosphatidic acid; lyso-PG, lyso-phosphatidylglycerol; lysyl-CL, lysyl-cardiolipin; lysyl-PG, lysyl-phosphatidylglycerol; PA, phosphatidic acid, PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PS, phosphatidylserine.