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. 2017 Aug 14;7:8036. doi: 10.1038/s41598-017-08176-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Apoptotic effects (%) of various concentrations of OipA on AGS and KATO III. AGS and KATO III were treated with different concentrations of OipA or heat-inactivated OipA or BSA as negative controls for 24 h and apoptosis was detected by FITC-Annexin V/Propidium Iodide staining. Representative flow-cytometrydot-plots showing the level of apoptosis in AGS (A) and KATO III (C). Bottom left: live cells, top left: necrosis, bottom right: early apoptosis, top right: late apoptosis. The results are expressed as the mean ± SD, (n = 3, three independent experiments) (B and D). Statistically significant differences with the control group are indicated with * (p < 0.05).