Table 2.
Ions Observed Following SCW Hydrolysis of VCFQYMDRGDR at 140 °C for 10 min
| m/z | z | Calculated mass (Da) | Measured mass (Da) | Peptide | ΔPPM |
|---|---|---|---|---|---|
| 463.8730 | 3 | 1388.5965 | 1388.5972 | VCFQYMDRGDR | 0.5 |
| 468.5323 | 3 | 1402.5758 | 1402.5751 | VCFQYMDRGDR (–H2O+2O) | -0.5 |
| 473.8640 | 3 | 1418.5707 | 1418.5702 | VCFQYMDRGDR (–H2O+3O) | –0.4 |
| 474.5359 | 3 | 1420.5863 | 1420.5859 | VCFQYMDRGDR (+2O) | –0.3 |
| 479.8675 | 3 | 1436.5812 | 1436.5807 | VCFQYMDRGDR (+3O) | –0.4 |
| 624.2444 | 2 | 1246.4747 | 1246.4742 | VCFQYMDRGD (–H2O+2O) | –0.3 |
| 632.2418 | 2 | 1262.4696 | 1262.4690 | VCFQYMDRGD (–H2O+3O) | –0.4 |
| 633.2498 | 2 | 1264.4852 | 1264.4850 | VCFQYMDRGD (+2O) | –0.1 |
| 635.2353 | 2 | 1268.4561 | 1268.4560 | VCFQYMDRGD (–H2O+2O+Na) | 0.0 |
| 641.2472 | 2 | 1280.4811 | 1280.4798 | VCFQYMDRGD (+3O) | –1.0 |
| 643.2327 | 2 | 1284.4510 | 1284.4508 | VCFQYMDRGD (–H2O+3O+Na) | –0.1 |
| 652.2381 | 2 | 1302.4625 | 1302.4616 | VCFQYMDRGD (+3O+Na) | –0.7 |
| 702.2950 | 2 | 1402.5758 | 1402.5754 | VCFQYMDRGDR (–H2O+2O) | –0.2 |
| 710.2924 | 2 | 1418.5707 | 1418.5702 | VCFQYMDRGDR (–H2O+3O) | –0.3 |
| 711.3004 | 2 | 1420.5863 | 1420.5862 | VCFQYMDRGDR (+2O) | –0.1 |
| 718.2898 | 2 | 1434.5656 | 1434.5650 | VCFQYMDRGDR (–H2O+4O) | –0.4 |
| 719.2999 | 2 | 1436.5812 | 1436.5852 | VCFQYMDRGDR (+3O) | 2.8 |
| 727.2950 | 2 | 1452.5761 | 1452.5754 | VCFQYMDRGDR (+4O) | –0.5 |
| 1229.4707 | 1 | 1228.4641 | 1228.4634 | VCFQYMDRGD (–2H2O+2O) | –0.6 |
| 1247.4811 | 1 | 1246.4747 | 1246.4738 | VCFQYMDRGD (–H2O+2O) | –0.7 |
| 1263.4760 | 1 | 1262.4696 | 1262.4687 | VCFQYMDRGD (–H2O+3O) | –0.7 |
| 1265.4926 | 1 | 1264.4852 | 1264.4853 | VCFQYMDRGD (+2O) | 0.1 |
| 1281.4868 | 1 | 1280.4811 | 1280.4795 | VCFQYMDRGD (+3O) | –1.2 |
| 1285.4578 | 1 | 1284.4510 | 1284.4505 | VCFQYMDRGD (–H2O+3O+Na) | –0.3 |