Skip to main content
. 2017 Jun 27;595(16):5573–5586. doi: 10.1113/JP274344

Figure 4. CLCNKB mutations around the selectivity filter.

Figure 4

A, locations of the mutations (highlighted in magenta) in or around the selectivity filter in oblique view with green subunit in front (PDB ID: 3ORG). B, Western blot of wild‐type versus mutant hClC‐Kb channel in the absence (+ EV) or presence (+ Barttin) of barttin co‐expression. C, I–V relationship curves (upper graph) and current at holding potential of −145 mV (lower graph) of wild‐type (WT) and mutant hClC‐Kb channels with barttin co‐expression (mean ± SEM, n ≥ 6 for each). ** P < 0.01 between wild‐type and each mutation by unpaired two‐tailed Student's t test.