Figure 3. Protein expression and phosphorylated/total protein ratio of mechanosensor complex before and after 14 days of sucrose intervention.
A, protein expression of VEGF‐R2, PECAM‐1 and VE‐cadherin. B, phosphorylated VEGF‐R2 (tyr1175), PECAM‐1 (tyr713) and VE‐cadherin (tyr731) to total protein ratio in muscle homogenates from musculus vastus lateralis at rest and after 20 min of passive leg movement. C, expression of VE‐CAD, VEGF‐R2 and PECAM‐1 in isolated primary skeletal muscle cells (SkMu Cell) and skeletal muscle tissue homogenate (SkMu Tissue). D, representative blots are shown from one subject in duplicates from rest (1) and after passive leg movement (2) under the control situation and from rest (3) and after passive leg movement (4) after the sucrose intervention. Data are presented as the mean ± SD (n = 12). *Significantly different compared to before the sucrose intervention during the condition of passive leg movement. **Overall significant effect of the sucrose intervention.
