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A, immunofluorescence images of TfR (green) and GLUT4 (magenta) before (left) and after 5 min of insulin stimulation (right, 100 nm) in isolated soleus (upper) and EDL (lower) myofibres. B, scatter plot analysis of GLUT4‐TfR co‐localization in soleus muscle shown in (A). C, changes in co‐localization coefficient of GLUT4 with TfR after 5 min of insulin stimulation (100 nm). * P < 0.05 (n = 5).
