Figure 1. FLDCs produce IFN‐I in response to Th2 Ag.

• A
  FLDC subsets were defined by expression of CD11c, CD45R (B220), CD11b, and CD24. pDCs were identified as CD45R⁺ CD11c^lo; cDCs are CD11c⁺ CD45R⁻ with cDC1 and cDC2 subsets.
• B, C
  IFN‐I production as measured by ELISA on supernatants from bulk cultures cultured in medium alone (M) or stimulated with 25 μg/ml soluble egg Ag from Schistosoma mansoni (SEA) (B, C), 5 μg/ml Salmonella typhimurium (St) (B), or 50 μg/ml house dust mite (HDM) extract (C).
• D
  Production of DC cytokines from bulk cultures following exposure to SEA or St.
• E–G
  IFN‐I production as measured by ELISA on supernatants from bulk cultures cultured in medium alone (M) or stimulated with 1:500 live or dead whole S. mansoni eggs (E), 0.5 μg/ml recombinant omega‐1 (F), or FACS‐isolated subsets after stimulation with SEA (G).
• H
  Percentage of IFNα6GFP⁺ DCs after stimulation with SEA, 1 μg/ml CpG, or HDM.
• I
  IFNα3 production by WT, Myd88 ^−/−, and Trif ^−/− bulk FLDCs after exposure to SEA.

Data information: Results are mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (one‐way ANOVA). Data from one of three or more experiments (n = 3 replicate wells per group).