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. 2017 Jul 17;36(16):2404–2418. doi: 10.15252/embj.201695345

Figure 4. Ifnar1 −/− FL‐cDC APC function is comparable to WT FL‐cDC in vitro .

Figure 4

  • A, B
    FL‐cDCs were sorted and co‐cultured with CFSE‐labeled OT‐II T cells, with either OVA protein (OVA) or peptide (pOVA), for 96 h. Proliferation was determined by flow cytometric analysis of CFSE dilution. The gray dashed line represents non‐proliferating OT‐II T cell controls.
  • C
    To assess Ag uptake and processing, WT or Ifnar1 −/− FLDCs were cultured with DQ‐OVA for 2 h at 37°C or 4°C and uptake assessed by flow cytometry.
  • D–F
    Sorted FL‐cDCs were cultured with eGFP− CD4+ T cells from KN2xIL‐13eGFP or KN2xIL‐10eGFP animals, with anti‐CD3, in the presence (+) or absence (−) of 20 ng/ml rIL‐4. IL‐4 (huCD2, D), IL‐10eGFP (E), or IL‐13eGFP (F) expression on CD4+ T cells was assessed by flow cytometry after 72 h of culture. Positive cells were expressed as a percentage of all CD4+ T cells.
Data information: Results are mean ± SEM. **P < 0.01, ***P < 0.001, ****P < 0.0001 (one‐way ANOVA). Data from one of three experiments (n = 4–5 wells per group).