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. 2017 Jul 10;36(16):2373–2389. doi: 10.15252/embj.201694912

Figure 2. Ccn1 is highly expressed in stiff regions of orthotopic E0771 tumors.

Figure 2

  1. Representative image of in situ hybridization for Ccn1 mRNA and collagen I and III fibers (Sirius red) of E0771 orthotopic tumors performed on consecutive sections showing that there are tumor regions with high and low expression of Ccn1. The right panels show a blood vessel positive for Ccn1 staining. Scale bar = 100 μm.
  2. Quantification of collagen I and III fiber content based on Sirius red staining (% of the measured area) showing that higher amounts of collagen fibers are found in regions of the tumor expressing high Ccn1 levels.
  3. Quantification of tumor stiffness performed by atomic force microscopy showing that higher stiffness is measured in peri‐necrotic regions (bona fide expressing high Ccn1 levels) of the tumor.
Data information: Significance according to two‐tailed Mann–Whitney test. Horizontal lines and error bars represent mean ± SEM. n = regions assessed from two tumors.