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. 2017 Aug 15;5:69. doi: 10.1186/s40425-017-0270-1

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Association of CpG with RHDV VLP. The ability of RHDV VLP to naturally associate with CpG was investigated by dialysis with 1 MDa tubing. a CpGs were detected by TBE acrylamide gel electrophoresis stained with Gel Green following dialysis. b Quanti-blue assay on supernatants detecting SEAP secreted from the reporter cell line, RAW-blue, following treatment with CpG associated with VLP. This treatment was repeated with murine BMDCs, detecting the median fluorescence intensity (MFI) of the activation markers c CD40, d CD80, e CD86 and f I-A/I-E by flow cytometry. Results are representative of two independent repeats. L = Hyperladder V, Dial = Dialysed. Statistical analysis performed using unpaired t-tests. NS = Non-significant, ** p < 0.01, *** p < 0.001, **** p < 0.0001