Fig. 4.

Comparison of Multiple VLP Vaccination Doses. Boosting of epitope-specific immune responses through multiple vaccination doses was investigated using a modified in vitro cytotoxicity assay. a Modified in vitro cytotoxicity assay, using a combination of CFSE and VPD to simultaneously monitor cytotoxicity against multiple target populations. b Cytotoxicity against survivin labelled target cells in mice vaccinated with VP60 on day 0 (1D), day 0 and 1 (2D) and days 0, 1 and 2 (3D). Results were comparable when calculated with respect to the SIINFEKL labelled population. Cytotoxicity was compared between mice vaccinated 1D, 2D or 3D with c SIIN.VP60 or d S.VP60 for their respective target populations to investigate whether an advantage in immunogenicity was gained through multiple dosages. Results are representative of three independent repeats. Statistical analysis performed using unpaired t-tests. NS = Non-significant, * p < 0.05, **** p < 0.0001