Figure 4. Robustness of behavioral rhythms is not impaired in Cx36−/− mice in constant light.
(A) Representative double-plotted actograms showing wheel-running activity of wild-type (left), Cx36+/− and Cx36−/− (right) mice in constant light after prior entrainment in light/dark. Yellow areas represent light. Increasing brightness of yellow areas represents increasing light intensities from 200 lux to 400 lux to 600 lux (14 days each). (B) Mean circadian period, amplitude, and total activity levels of circadian behavioral rhythms from wild-type (black) and Cx36−/− (red) mice in LD and in LL (yellow areas). Data are shown as mean ± SEM. PERIOD: Interaction: F3,11 = 0.1389, p = 0.9360; Light conditions: F3,11 = 2.119, p = 0.1167; Genotype: F1,11 = 0.4167, p = 0.5318; post hoc: not significant. AMPLITUDE: Interaction: F3,12 = 0.3861, p = 0.7636; Light conditions: F3,12 = 32.51, p < 0.0001; Genotype: F1,12 = 0.8797, p = 0.3668; post hoc: not significant. ACTIVITY LEVELS: Interaction: F3,12 = 0.08383, p = 0.9684; Light conditions: F3,12 = 48.27, p < 0.0001; Genotype: F1,12 = 1.366, p = 0.2652; post hoc: not significant (2-way repeated measurement ANOVA); wild-type: n = 7/8; Cx36−/−: n = 6.