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. 2017 Jul 1;8(10):1774–1785. doi: 10.7150/jca.19732

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© Ivyspring International Publisher

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Expression of CD44v in lung cancer cell lines. A) Several lung cancer cell lines, 11 adenocarcinoma (H1975, H1650, PC9, A549, H441, H358, H522, SK-LU1, MCF7, Calu-3, HCC827), 3 squamous cell carcinoma (SK-MES1, H520, SW900), 1 neuroendocrine (H1770) and 1 epidermoid (Calu-1) cancer cell line, in addition to the NHBE and BEAS2B non-cancer cell lines, were examined for CD44v v9 expression using RT-PCR. Cytospun slides containing cells from the H1650 and HCC827 (expressed CD44v RNA) and A549 (did not express CD44v RNA) cell lines were stained with anti-CD44v-specific antibody (B-D) or panCD44 antibody (E). H1650 and HCC827 cells, but not A549 cells, expressed CD44v. A549 cells expressed other isoform(s). Similar results were seen by flow cytometry (F-H). Scale bars: 50 μm.