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Examining CD44vhigh cells for CSC characteristics. CD44vhigh and CD44vlow cells were sorted from H1650, and panCD44high and panCD44low cells were sorted from A549. (A, B) In vitro cell proliferation MTS assay. (C) Chemotherapeutic-resistance test: viable cells are in the left lower quadrant. (D, E) Xenograft tumor formation in immunocompromised mice, H1650: n=2 and A549: n=3.
