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. 2017 Jul 3;8(10):1892–1899. doi: 10.7150/jca.17638

Figure 6.

Figure 6

TBK1 promote bladder tumorigenesis via Akt signaling. (A). T24 cells were transfected with Flag-tagged TBK1. Twenty-four hours later, cell extracts were analyzed for Akt phosphorylation. (B). The nonspecific control (NC) or TBK1 siRNA were transfected into T24 cells. Fourty-eight hours later, cell extracts were analyzed for Akt phosphorylation. (C and D). Cells knocking down TBK1 were treated with perifosine, a PI3K/AKT inhibitor. The cell proliferation and migration were determined by CCK-8 (C) and transwell assay (D).