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. Author manuscript; available in PMC: 2017 Aug 15.
Published in final edited form as: Structure. 2017 Apr 20;25(5):773–782.e5. doi: 10.1016/j.str.2017.03.015

Figure 1. N-Glycan-Binding Assays to BanLec and Inhibition Assays.

Figure 1

(A) HPLC depletion assay of released gp120 N-glycans incubated with 0, 1, 5, 10, 20, and 50 μM BanLec.

(B) Inhibition of HIV pseudovirus strains JRCSF and BaL expressed with and without kifunensine (kif).

(C) Native mass spectra of BanLec with different concentrations of Man9 (0, 2.5, 7.5, 15, 30, and 50 μM).

(D) Kds determined from MS experiments binding Man7, Man8, and Man9 to BanLec, and calculated from peak intensities.