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PEAMT expression and Cho levels in leaves of tobacco primary transformants grown in culture. (A) Immunoblot analysis of recombinant spinach PEAMT protein in leaf extracts of eight individual PEAMT+ transformants (tracks 1–8) and an empty-vector control (V); salinized spinach (SS) is included for comparison. Tracks contained 50 μg of protein. (B) Free Cho levels in the same plants.
