Table 1.
PEAMT activity and levels of Cho and its metabolites in leaves of transgenic tobacco plants expressing spinach PEAMT
| Transgenic line | Growth conditions | PEAMT activity, pkat mg−1 protein | Cho moieties, μmol
g−1FW
|
||||
|---|---|---|---|---|---|---|---|
| Cho | P-Cho | Ptd-Cho | GlyBet | Total | |||
| Vector | Culture | <0.1 | 0.11 | 0.11 | 1.56 | 0.02 | 1.80 |
| 1 | Culture | 5.00 | 4.78 | 0.63 | 1.42 | 0.45 | 7.28 |
| 4 | Culture | 4.63 | 5.03 | 0.94 | 1.62 | 0.41 | 8.00 |
| 5 | Culture | 3.41 | 5.27 | 0.35 | 1.38 | 0.62 | 7.62 |
| Vector (YL) | Greenhouse | — | 0.13 | 0.08 | — | 0.04 | — |
| 1 (YL) | Greenhouse | — | 5.21 | 0.23 | — | 1.81 | — |
| 1 (OL) | Greenhouse | — | 5.75 | 0.19 | — | 1.16 | — |
Three PEAMT+ lines and an empty-vector control were cultured in vitro. Line 1 and the empty-vector control were also grown in the greenhouse for 10 weeks, after which a half-expanded leaf (YL) and the third fully expanded leaf (OL) were analyzed. All data are values for individual plants. P-Cho, phosphocholine; Ptd-Cho, phosphatidylcholine.