Fig. 8.

Effect of wounding of host leaves on pycnidiation by Z. tritici IPO323 under six humidity regimes. Leaves were gently abraded prior to inoculation with 10⁶ cfu/ml suspensions of Z. tritici. Control leaves were inoculated, but not abraded. Plants were then maintained under six different humidity regimes (#1 – lowest; #6 – highest). Inoculated leaves were examined from 14 dpi onwards, and the percentage of inoculated leaves bearing pycnidia determined on each day. Data are means of two independent experiments; error bars show SE. Within each time point, two-way ANOVAs were carried out. In each case, humidity, wounding, and the interaction between these treatments were all significant predictors of pycnidiation (P-values above graphs; black text = humidity, grey text = wounding, ^* = interaction term).